2VBX
Feast or famine regulatory protein (Rv3291c)from M. tuberculosis complexed with L-Phenylalanine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU300 |
Temperature [K] | 298 |
Detector technology | AREA DETECTOR |
Detector | MARRESEARCH |
Spacegroup name | P 42 21 2 |
Unit cell lengths | 100.899, 100.899, 99.286 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 71.430 - 2.750 |
R-factor | 0.228 |
Rwork | 0.226 |
R-free | 0.27000 |
Structure solution method | MIRAS |
Starting model (for MR) | NONE |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | SOLVE |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.900 |
High resolution limit [Å] | 2.750 | 2.750 |
Rmerge | 0.110 | 0.570 |
Number of reflections | 13598 | |
<I/σ(I)> | 13.7 | 2.9 |
Completeness [%] | 98.6 | 99.7 |
Redundancy | 5.5 | 5.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 |