2VBO
Molecular basis of human XPC gene recognition and cleavage by engineered homing endonuclease heterodimers
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 45.993, 67.950, 76.629 |
| Unit cell angles | 90.00, 90.10, 90.00 |
Refinement procedure
| Resolution | 39.470 - 1.800 |
| R-factor | 0.153 |
| Rwork | 0.150 |
| R-free | 0.22500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1g9z |
| RMSD bond length | 0.011 |
| RMSD bond angle | 2.077 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 39.460 |
| High resolution limit [Å] | 1.800 |
| Rmerge | 0.070 |
| Number of reflections | 41668 |
| Completeness [%] | 94.6 |
| Redundancy | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.5 | pH 4.5 |
