2VAL
Crystal structure of an Escherichia coli tRNAGly microhelix at 2.0 Angstrom resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X13 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X13 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARRESEARCH |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 35.348, 35.348, 130.821 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 17.670 - 2.000 |
| Rwork | 0.241 |
| R-free | 0.31800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.030 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.079 | 0.293 |
| Number of reflections | 6908 | |
| <I/σ(I)> | 34 | 5.8 |
| Completeness [%] | 99.1 | 83.4 |
| Redundancy | 9.4 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 50 MM SODIUM CACODYLATE, PH 6.0, 10 MM SPERMINE, 10 MM MAGNESIUM SULFATE, 2.0 M AMMONIUM SULFATE, 0.5 MM RNA, ROOM TEMPERATUE. |
