2V9D
Crystal Structure of YagE, a prophage protein belonging to the dihydrodipicolinic acid synthase family from E. coli K12
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-12-04 |
| Detector | ADSC CCD |
| Wavelength(s) | 0.9794 , 0.9796 , 0.9762 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 56.279, 149.806, 78.967 |
| Unit cell angles | 90.00, 108.42, 90.00 |
Refinement procedure
| Resolution | 36.350 - 2.150 |
| R-factor | 0.168 |
| Rwork | 0.166 |
| R-free | 0.21800 |
| Structure solution method | MAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.682 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.230 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rmerge | 0.060 | 0.140 |
| Number of reflections | 67391 | |
| <I/σ(I)> | 19 | |
| Completeness [%] | 99.9 | 99.7 |
| Redundancy | 7.5 | 7.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.5 | 100MM BIS-TRIS PH 5.5, 200MM MGCL2, 12% PEG 3350 |
