2V9A

Structure of Citrate-free Periplasmic Domain of Sensor Histidine Kinase CitA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MPG/DESY, HAMBURG BEAMLINE BW6
Synchrotron site	MPG/DESY, HAMBURG
Beamline	BW6
Temperature [K]	100
Detector technology	CCD
Collection date	2006-03-08
Detector	MARRESEARCH
Spacegroup name	I 4
Unit cell lengths	64.163, 64.163, 144.746
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.663 - 2.000
Rwork	0.212
R-free	0.27830
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2j80
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	2.100
High resolution limit [Å]	2.000	2.000
Rmerge	0.050	0.190
Number of reflections	19151
<I/σ(I)>	16.7
Completeness [%]	97.0	84.1
Redundancy	5.49	1.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	HANGING DROP, VAPOUR DIFFUSION, RESERVOIR: 20MM HEPES,PH 7.5,0.63M NAH2PO4,0.63M KH2PO4, PROTEIN SOLUTION: 15 MG/ML,MIXING RATIO 1:1,TEMPERATURE 293K