2V90
Crystal structure of the 3rd PDZ domain of intestine- and kidney- enriched PDZ domain IKEPP (PDZD3)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-04-16 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 48.150, 97.950, 59.500 |
| Unit cell angles | 90.00, 99.59, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.000 |
| R-factor | 0.196 |
| Rwork | 0.193 |
| R-free | 0.25600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1g9o |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.475 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.3.0034) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.700 | 2.100 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.070 | 0.280 |
| Number of reflections | 36079 | |
| <I/σ(I)> | 9.8 | 3.7 |
| Completeness [%] | 97.8 | 94.7 |
| Redundancy | 3.6 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.5 | 2M (NH4)2SO4, 0.1M BIS-TRIS, PH=5.5 |
