2V89

Crystal structure of RAG2-PHD finger in complex with H3K4me3 peptide at 1.1A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2006-08-04
Detector	MARRESEARCH
Spacegroup name	C 1 2 1
Unit cell lengths	75.978, 46.807, 56.808
Unit cell angles	90.00, 106.78, 90.00

Refinement procedure

Resolution	50.000 - 1.100
R-factor	0.1227
R-free	0.15510
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2v83
RMSD bond length	0.016
RMSD bond angle	0.033
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	SHELXL-97

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.140
High resolution limit [Å]	1.100	1.100
Rmerge	0.060	0.360
Number of reflections	75608
<I/σ(I)>	28.3	2.4
Completeness [%]	93.8	67.5
Redundancy	3.6	2.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	CRYSTALS OF COMPLEX WERE OBTAINED AT 3 MG/ML PROTEIN CONCENTRATION AND 1:1.5 MOLAR RATIO OF PROTEIN TO PEPTIDE USING HANGING DROP AND VAPOR DIFFUSION TECHNIQUE AT 293K. PRECIPITANT SOLUTION: 26% PEG 3350, 0.18M POTASSIUM THIOCYANATE.