2V89
Crystal structure of RAG2-PHD finger in complex with H3K4me3 peptide at 1.1A resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-08-04 |
Detector | MARRESEARCH |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 75.978, 46.807, 56.808 |
Unit cell angles | 90.00, 106.78, 90.00 |
Refinement procedure
Resolution | 50.000 - 1.100 |
R-factor | 0.1227 |
R-free | 0.15510 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2v83 |
RMSD bond length | 0.016 |
RMSD bond angle | 0.033 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | SHELXL-97 |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.140 |
High resolution limit [Å] | 1.100 | 1.100 |
Rmerge | 0.060 | 0.360 |
Number of reflections | 75608 | |
<I/σ(I)> | 28.3 | 2.4 |
Completeness [%] | 93.8 | 67.5 |
Redundancy | 3.6 | 2.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | CRYSTALS OF COMPLEX WERE OBTAINED AT 3 MG/ML PROTEIN CONCENTRATION AND 1:1.5 MOLAR RATIO OF PROTEIN TO PEPTIDE USING HANGING DROP AND VAPOR DIFFUSION TECHNIQUE AT 293K. PRECIPITANT SOLUTION: 26% PEG 3350, 0.18M POTASSIUM THIOCYANATE. |