2V87
Crystal structure of RAG2-PHD finger in complex with H3R2me2sK4me3 peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-08-17 |
| Detector | RIGAKU-MSC |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 40.407, 46.630, 57.005 |
| Unit cell angles | 90.00, 95.13, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.800 |
| R-factor | 0.181 |
| Rwork | 0.181 |
| R-free | 0.20810 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2v83 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.811 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.060 | 0.210 |
| Number of reflections | 19094 | |
| <I/σ(I)> | 25.5 | 3.9 |
| Completeness [%] | 96.5 | 87.9 |
| Redundancy | 2.6 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | VAPOR DIFFUSION. HANGING DROP. 22% PEG 3350, 120-240 MM OF POTASSIUM FORMATE. TEMPERATURE 293K |
