2V7U
X-ray crystal structure of 5'-fluorodeoxyadenosine synthase s158g mutant complexed with s-adenosylmethionine and chloride ion
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-05-28 |
| Detector | RIGAKU IMAGE PLATE |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 76.224, 129.377, 183.080 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.700 - 2.000 |
| R-factor | 0.166 |
| Rwork | 0.164 |
| R-free | 0.20400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1rqp |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.352 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.100 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.080 | 0.360 |
| Number of reflections | 55867 | |
| <I/σ(I)> | 17.8 | 3.3 |
| Completeness [%] | 96.0 | 98.6 |
| Redundancy | 4.9 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.6 | 0.1M CITRATE-PHOSPHATE BUFFER PH4.6, 20%(W/V) PEG1000, 0.2M LICL |
