2V7R
Crystal structure of a human tRNAGly microhelix at 1.2 Angstrom resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X13 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X13 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARRESEARCH |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 37.120, 37.491, 30.379 |
| Unit cell angles | 90.00, 113.02, 90.00 |
Refinement procedure
| Resolution | 14.080 - 1.200 |
| R-factor | 0.188 |
| Rwork | 0.187 |
| R-free | 0.20800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.712 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.220 |
| High resolution limit [Å] | 1.200 | 1.200 |
| Rmerge | 0.050 | 0.240 |
| Number of reflections | 11547 | |
| <I/σ(I)> | 18.1 | |
| Completeness [%] | 95.3 | 93.7 |
| Redundancy | 7.2 | 7.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 40 MM SODIUM CACODYLATE, PH 6.0, 12 MM SPERMINE TETRA-HCL, 12 MM SODIUM CHLORIDE, 80 MM POTASSIUM CHLORIDE, 10% (V/V) MPD, EQUILIBRATED AGAINST 35% (V/V) MPD AT ROOM TEMPERATURE |
