2V7E
Crystal structure of coactivator-associated arginine methyltransferase 1 (CARM1), unliganded
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-12-11 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 74.146, 98.018, 206.915 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.690 - 2.700 |
| R-factor | 0.23 |
| Rwork | 0.228 |
| R-free | 0.27600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ori |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.140 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.850 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.070 | 0.210 |
| Number of reflections | 19941 | |
| <I/σ(I)> | 8.5 | 5.1 |
| Completeness [%] | 99.8 | 99.4 |
| Redundancy | 5.8 | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 1.6M DI-AMMONIUM HYDROGENPHOSPHATE, 100MM HEPES PH 7.5 |
