2V68
Crystal structure of Chlamydomonas reinhardtii Rubisco with large- subunit mutations V331A, T342I
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I711 |
| Synchrotron site | MAX II |
| Beamline | I711 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-09-26 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 120.421, 178.236, 122.758 |
| Unit cell angles | 90.00, 117.72, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.300 |
| R-factor | 0.174 |
| Rwork | 0.172 |
| R-free | 0.20300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1gk8 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.230 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.290 |
| High resolution limit [Å] | 2.300 | 2.250 |
| Rmerge | 0.160 | 0.400 |
| Number of reflections | 1285694 | |
| <I/σ(I)> | 10.8 | 3.1 |
| Completeness [%] | 99.2 | 88.6 |
| Redundancy | 20.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | pH 7.5 |
