2V67
Crystal structure of Chlamydomonas reinhardtii Rubisco with a large- subunit supressor mutation T342I
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-05-15 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 120.094, 178.558, 122.495 |
| Unit cell angles | 90.00, 117.83, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.000 |
| R-factor | 0.176 |
| Rwork | 0.174 |
| R-free | 0.20800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1gk8 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.140 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.030 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.130 | 0.690 |
| Number of reflections | 272649 | |
| <I/σ(I)> | 7.5 | 1.3 |
| Completeness [%] | 89.2 | 89.9 |
| Redundancy | 17.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | pH 7.5 |
