2V5J
Apo Class II aldolase HpcH
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-05-02 |
| Detector | MARRESEARCH |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 128.920, 128.920, 175.300 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 40.790 - 1.600 |
| R-factor | 0.174 |
| Rwork | 0.173 |
| R-free | 0.20700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1dxe |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.471 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.800 | 1.640 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.070 | 0.710 |
| Number of reflections | 73671 | |
| <I/σ(I)> | 33.6 | 2.5 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 10.9 | 10.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5 | 0.4 M AMMONIUM DIHYDROGEN PHOSPHATE, 30% (V/V) GLYCEROL, pH 5 |
