2V53
Crystal structure of a SPARC-collagen complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX10.1 |
| Synchrotron site | SRS |
| Beamline | PX10.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-10-24 |
| Detector | MARRESEARCH |
| Spacegroup name | P 42 21 2 |
| Unit cell lengths | 89.790, 89.790, 127.100 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.842 - 3.200 |
| R-factor | 0.2609 |
| Rwork | 0.254 |
| R-free | 0.32020 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1nub |
| RMSD bond length | 0.000 |
| RMSD bond angle | 1.410 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 3.370 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.080 | 0.270 |
| Number of reflections | 9046 | |
| <I/σ(I)> | 16.5 | 7.4 |
| Completeness [%] | 99.6 | 100 |
| Redundancy | 8.7 | 8.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.5 | pH 5.5 |
