2V52

Structure of MAL-RPEL2 complexed to G-actin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	CCD
Collection date	2007-10-11
Detector	ADSC CCD
Spacegroup name	P 21 21 21
Unit cell lengths	54.750, 55.440, 138.390
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.352 - 1.450
R-factor	0.149
Rwork	0.147
R-free	0.18780
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.060
RMSD bond angle	2.840
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.530
High resolution limit [Å]	1.450	1.450
Rmerge	0.070	0.550
Number of reflections	74958
<I/σ(I)>	6	1.2
Completeness [%]	99.3	96
Redundancy	5.2	2.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1