2V4M
The isomerase domain of human glutamine-fructose-6-phosphate transaminase 1 (GFPT1) in complex with fructose 6-phosphate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I711 |
| Synchrotron site | MAX II |
| Beamline | I711 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-10-27 |
| Detector | MARRESEARCH |
| Spacegroup name | H 3 |
| Unit cell lengths | 178.824, 178.824, 157.113 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 54.890 - 2.290 |
| R-factor | 0.168 |
| Rwork | 0.167 |
| R-free | 0.18500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1moq |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.094 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0035) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 55.000 | 2.390 |
| High resolution limit [Å] | 2.290 | 2.290 |
| Rmerge | 0.050 | 0.210 |
| Number of reflections | 282618 | |
| <I/σ(I)> | 22.6 | 5.1 |
| Completeness [%] | 99.2 | 96.8 |
| Redundancy | 3.4 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
