2V4E
A non-cytotoxic DsRed variant for whole-cell labeling
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-BM-C |
| Synchrotron site | APS |
| Beamline | 14-BM-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-06-15 |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 115.526, 122.619, 164.851 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.400 |
| R-factor | 0.223 |
| Rwork | 0.220 |
| R-free | 0.27100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2vae |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.434 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0053) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.140 | 0.610 |
| Number of reflections | 92897 | |
| <I/σ(I)> | 14.3 | 2.2 |
| Completeness [%] | 98.0 | 84.8 |
| Redundancy | 6.6 | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 298 | 100 MM BIS-TRIS, PH 5.5 200 MM AMMONIUM SULFATE, 16% PEG8000, VAPOR DIFFUSION, SITTING DROP, 298K |
