2V4B
Crystal Structure of Human ADAMTS-1 catalytic Domain and Cysteine- Rich Domain (apo-form)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-09-01 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 51.579, 64.396, 113.524 |
| Unit cell angles | 90.00, 90.91, 90.00 |
Refinement procedure
| Resolution | 40.260 - 2.000 |
| R-factor | 0.222 |
| Rwork | 0.220 |
| R-free | 0.26300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.163 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.260 | 2.110 |
| High resolution limit [Å] | 1.410 | 2.000 |
| Rmerge | 0.110 | 0.480 |
| Number of reflections | 50457 | |
| <I/σ(I)> | 4.17 | 1.06 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 3.64 | 3.63 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 0.2-0.6M SODIUM ACETATE, 0.05M CADMIUM SULPHATE, 0.1M HEPES PH 7.0, 12-22% GLYCEROL |
