2V43
Crystal structure of RseB: a sensor for periplasmic stress response in E. coli
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-09-07 |
| Detector | ADSC CCD |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 98.600, 200.700, 109.730 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.370 |
| R-factor | 0.231 |
| Rwork | 0.228 |
| R-free | 0.27800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | LARGE DOMAIN FROM P4212 DATASET |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.697 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.500 |
| High resolution limit [Å] | 2.400 | 2.370 |
| Rmerge | 0.110 | 0.580 |
| Number of reflections | 40893 | |
| <I/σ(I)> | 12.6 | 2.4 |
| Completeness [%] | 95.2 | 89.4 |
| Redundancy | 3.7 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | PROTEIN WAS CRYSTALLIZED FROM 0.2 M MAGNESIUM CHLORIDE HEXAHYDRATE, 0.1 M TRIS-HCL PH 8.5, 25%(W/V) POLYETHYLENE GLYCOL 3350 AND 10 MM L-CYSTEINE |
