2V3U
Structure of the ligand-binding core of the ionotropic glutamate receptor-like GluRdelta2 in complex with D-serine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-01-14 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 |
| Unit cell lengths | 36.801, 40.307, 44.393 |
| Unit cell angles | 66.88, 79.31, 86.46 |
Refinement procedure
| Resolution | 40.190 - 1.740 |
| R-factor | 0.2 |
| Rwork | 0.197 |
| R-free | 0.25000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2v3t |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.526 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0016) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.820 |
| High resolution limit [Å] | 1.730 | 1.730 |
| Rmerge | 0.040 | 0.260 |
| Number of reflections | 23508 | |
| <I/σ(I)> | 12 | 2.1 |
| Completeness [%] | 92.3 | 70.1 |
| Redundancy | 3.8 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | HANGING DROP VAPOR DIFFUSION. 20% PEG4000, 0.1 M CACODYLATE PH 6.5, 0.2 M SODIUM THIOCYANATE |
