2V3E
acid-beta-glucosidase with N-nonyl-deoxynojirimycin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM14 |
Synchrotron site | ESRF |
Beamline | BM14 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-11-15 |
Detector | MARRESEARCH |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 67.998, 97.646, 82.348 |
Unit cell angles | 90.00, 102.75, 90.00 |
Refinement procedure
Resolution | 23.620 - 2.000 |
R-factor | 0.163 |
Rwork | 0.160 |
R-free | 0.22000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2v3f |
RMSD bond length | 0.016 |
RMSD bond angle | 1.550 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 23.620 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.080 | 0.340 |
Number of reflections | 59655 | |
<I/σ(I)> | 10.27 | 2 |
Completeness [%] | 88.9 | 52.2 |
Redundancy | 2 | 2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.5 | 0.2 M NH4COOCH3, 0.1 M HEPES PH 7.5, 25% (W/V) PEG 3350 |