2V2H
The A178L mutation in the C-terminal hinge of the flexible loop-6 of triosephosphate isomerase (TIM) induces a more closed conformation of this hinge region in dimeric and monomeric TIM
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7A |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-03-30 |
| Detector | MARRESEARCH |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 68.260, 117.990, 81.770 |
| Unit cell angles | 90.00, 95.84, 90.00 |
Refinement procedure
| Resolution | 25.000 - 1.180 |
| R-factor | 0.1394 |
| R-free | 0.18680 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ml1 |
| RMSD bond length | 0.022 |
| RMSD bond angle | 0.041 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 1.190 |
| High resolution limit [Å] | 1.180 | 1.180 |
| Rmerge | 0.060 | 0.490 |
| Number of reflections | 210261 | |
| <I/σ(I)> | 17.01 | 3.7 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 5.2 | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.5 | 0.1 M CITRIC ACID PH 5.5, 20% PEG 6K, 3% TERT.BUTANOL |
