2V2D
The A178L mutation in the C-terminal hinge of the flexible loop-6 of triosephosphate isomerase (TIM) induces a more closed conformation of this hinge region in dimeric and monomeric TIM
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7B |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-11-26 |
| Detector | MARRESEARCH |
| Spacegroup name | P 63 |
| Unit cell lengths | 95.230, 95.230, 48.820 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.220 - 2.300 |
| R-factor | 0.204 |
| Rwork | 0.201 |
| R-free | 0.27000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ml1 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.458 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.3.0028) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.350 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.080 | 0.220 |
| Number of reflections | 11321 | |
| <I/σ(I)> | 24.45 | 13.03 |
| Completeness [%] | 99.2 | 99.9 |
| Redundancy | 12.1 | 12.44 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.2 | 1.75 M (NH4)2PO4, PH 8.2 |
