Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL40B2 |
Synchrotron site | SPring-8 |
Beamline | BL40B2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-03-04 |
Detector | ADSC CCD |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 151.781, 64.146, 68.018 |
Unit cell angles | 90.00, 94.70, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.050 |
R-factor | 0.254 |
Rwork | 0.248 |
R-free | 0.30800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1om2 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.226 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.150 |
High resolution limit [Å] | 2.050 | 2.050 |
Rmerge | 0.050 | 0.400 |
Number of reflections | 34455 | |
<I/σ(I)> | 13.67 | 2.47 |
Completeness [%] | 93.0 | 64.1 |
Redundancy | 4.51 | 1.96 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7 | PEG6000, AMMONIUM CHROLIDE, HEPES, PH 7.0, VAPOR DIFFUSION |