2V1O
Crystal structure of N-terminal domain of acyl-CoA thioesterase 7
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Detector | RIGAKU R-AXIS IV |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 55.741, 125.531, 81.780 |
| Unit cell angles | 90.00, 95.39, 90.00 |
Refinement procedure
| Resolution | 29.290 - 1.780 |
| R-factor | 0.212 |
| Rwork | 0.210 |
| R-free | 0.24400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.167 |
| Data reduction software | CrystalClear |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.280 | 1.850 |
| High resolution limit [Å] | 1.780 | 1.780 |
| Rmerge | 0.050 | 0.360 |
| Number of reflections | 101344 | |
| <I/σ(I)> | 13.8 | 3.7 |
| Completeness [%] | 95.5 | 76.3 |
| Redundancy | 5.87 | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.2 M SODIUM POTASSIUM TARTRATE, 15% PEG 2000 MONO METHYL ETHER |
