2V1M
Crystal structure of Schistosoma mansoni glutathione peroxidase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-05-05 |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 41.397, 60.625, 62.542 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 62.500 - 1.000 |
| R-factor | 0.176 |
| Rwork | 0.175 |
| R-free | 0.18400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2obi |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.513 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.380 | 1.050 |
| High resolution limit [Å] | 1.000 | 1.000 |
| Rmerge | 0.100 | 0.310 |
| Number of reflections | 81445 | |
| <I/σ(I)> | 17.8 | 2.4 |
| Completeness [%] | 100.0 | 95.3 |
| Redundancy | 6.3 | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 0.2M LISULFATE, 0.2M NAACETATE, PH 4.5, 24% PEG 8000 |
