2V18
Crystal structure of the T. thermophilus dodecin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | ENRAF-NONIUS FR591 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-12-28 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 67.481, 98.038, 137.595 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 24.760 - 2.590 |
| R-factor | 0.22 |
| Rwork | 0.219 |
| R-free | 0.24400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1mog |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.277 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.800 | 2.730 |
| High resolution limit [Å] | 2.600 | 2.590 |
| Rmerge | 0.080 | 0.240 |
| Number of reflections | 22085 | |
| <I/σ(I)> | 19 | 3.9 |
| Completeness [%] | 76.0 | |
| Redundancy | 2.75 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.2 M SODIUM CITRATE, 0.1 M TRIS/HCL, PH 7.5, 25% PEG 400 |
