2V09
SENS161-164DSSN mutant of Bacillus subtilis Oxalate Decarboxylase OxdC
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX14.1 |
| Synchrotron site | SRS |
| Beamline | PX14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-11-23 |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 154.602, 154.602, 122.957 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.220 - 1.800 |
| R-factor | 0.143 |
| Rwork | 0.141 |
| R-free | 0.17300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1uw8 |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.660 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.190 | 1.820 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.090 | 0.310 |
| Number of reflections | 49647 | |
| <I/σ(I)> | 15.92 | 1.51 |
| Completeness [%] | 95.3 | 72.8 |
| Redundancy | 6.47 | 2.02 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 8-15% PEG 8000, 0.1 M TRIS PH8.5, 0-15% XYLITOL |
