2UZS
A transforming mutation in the pleckstrin homology domain of AKT1 in cancer (AKT1-PH_E17K)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-07-17 |
| Detector | ADSC CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 79.299, 32.711, 41.983 |
| Unit cell angles | 90.00, 116.67, 90.00 |
Refinement procedure
| Resolution | 37.530 - 2.460 |
| R-factor | 0.211 |
| Rwork | 0.207 |
| R-free | 0.28600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1unq |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.542 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.500 | 2.540 |
| High resolution limit [Å] | 2.460 | 2.450 |
| Rmerge | 0.080 | 0.130 |
| Number of reflections | 3647 | |
| <I/σ(I)> | 13.5 | 9.5 |
| Completeness [%] | 97.0 | 88.9 |
| Redundancy | 2 | 1.54 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | GROWN FROM HANGING DROPS IN 0.1 M HEPES PH 7.5 AND 1.4 M SODIUM CITRATE, OR 0.1 M ACETATE PH 4.6, 0.2 M AMMONIUM ACETATE AND 15%-30% PEG 3350, |
