2UYY

Structure of the cytokine-like nuclear factor n-pac

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	CCD
Collection date	2007-03-03
Detector	MARRRESEARCH
Spacegroup name	H 3
Unit cell lengths	134.180, 134.180, 261.463
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	50.000 - 2.500
R-factor	0.172
Rwork	0.170
R-free	0.21100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1vpd 2i9p
RMSD bond length	0.012
RMSD bond angle	1.286
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.700	2.590
High resolution limit [Å]	2.500	2.500
Rmerge	0.150	0.540
Number of reflections	60779
<I/σ(I)>	8.93	2.35
Completeness [%]	99.8	98.3
Redundancy	5.61	4.42

Crystallization Conditions

crystal ID	method	pH	temperature	details
1				20% PEG3350 0.20 M KSCN