2UYA
DEL162-163 mutant of Bacillus subtilis Oxalate Decarboxylase OxdC
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-09-23 |
| Detector | ADSC CCD |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 154.857, 154.857, 123.274 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 58.930 - 2.000 |
| R-factor | 0.13 |
| Rwork | 0.129 |
| R-free | 0.16600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1uw8 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.499 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 58.930 | 2.020 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.100 | 0.290 |
| Number of reflections | 38270 | |
| <I/σ(I)> | 22.21 | 4.55 |
| Completeness [%] | 99.9 | 99.1 |
| Redundancy | 8.87 | 6.99 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 8-15% PEG 8000, 0.1 M TRIS PH8.5, 0-15% XYLITOL |
