2UXL
X-ray high resolution structure of the photosynthetic reaction center from Rb. sphaeroides at pH 10 in the neutral state, 2nd dataset
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-08-13 |
| Detector | MARRESEARCH |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 139.005, 139.005, 183.554 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 119.520 - 2.880 |
| R-factor | 0.178 |
| Rwork | 0.176 |
| R-free | 0.22000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1pcr |
| RMSD bond length | 0.017 |
| RMSD bond angle | 2.339 |
| Data reduction software | HKL |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.880 |
| High resolution limit [Å] | 2.850 | 2.850 |
| Rmerge | 0.100 | 0.400 |
| Number of reflections | 38279 | |
| <I/σ(I)> | 17.2 | 2.3 |
| Completeness [%] | 81.3 | 71.4 |
| Redundancy | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 10 | pH 10 |
