2UXK
X-ray high resolution structure of the photosynthetic reaction center from Rb. sphaeroides at pH 10 in the charge-separated state
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | X11 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2002-04-23 |
Detector | MARRESEARCH |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 140.020, 140.020, 235.875 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 119.520 - 2.310 |
R-factor | 0.191 |
Rwork | 0.190 |
R-free | 0.21900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1pcr |
RMSD bond length | 0.012 |
RMSD bond angle | 2.206 |
Data reduction software | HKL |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | REFMAC (5.1.24) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.220 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.050 | 0.630 |
Number of reflections | 112221 | |
<I/σ(I)> | 23.2 | 1.12 |
Completeness [%] | 93.8 | 60.4 |
Redundancy | 4.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 10 | pH 10 |