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2UXC

Crystal structure of an extended tRNA anticodon stem loop in complex with its cognate mRNA UCGU in the context of the Thermus thermophilus 30S subunit.

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsESRF BEAMLINE ID14-4
Synchrotron siteESRF
BeamlineID14-4
Temperature [K]100
Detector technologyCCD
Collection date2005-10-28
DetectorMARRESEARCH
Spacegroup nameP 41 21 2
Unit cell lengths401.822, 401.822, 175.104
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution48.280 - 2.900
R-factor0.217
Rwork0.217
R-free0.25500
Structure solution methodMOLECULAR REPLACEMENT
RMSD bond length0.006
RMSD bond angle1.200
Data reduction softwareXDS
Data scaling softwareXDS
Phasing softwareCNS
Refinement softwareCNS (1.1)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]50.0003.000
High resolution limit [Å]2.9002.900
Rmerge0.0900.580
Number of reflections1251329
<I/σ(I)>12.182.41
Completeness [%]95.989.2
Redundancy4.24
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP6.5277MPD, AMMONIUM CHLORIDE, POTASSIUM CHLORIDE, MAGNESIUM ACETATE, SODIUM CACODYLATE, PH 6.5, VAPOR DIFFUSION, HANGING DROP AT 277K

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