2UX9
Crystal structure of the T. thermophilus dodecin R65A mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE BW7A |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | BW7A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-04-01 |
Detector | MARRESEARCH |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 67.585, 67.585, 169.188 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 14.880 - 1.400 |
R-factor | 0.17 |
Rwork | 0.170 |
R-free | 0.19300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1mog |
RMSD bond length | 0.008 |
RMSD bond angle | 1.427 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 14.880 | 1.440 |
High resolution limit [Å] | 1.400 | 1.400 |
Rmerge | 0.080 | 0.300 |
Number of reflections | 87239 | |
<I/σ(I)> | 19.7 | 4.2 |
Completeness [%] | 98.0 | 96.2 |
Redundancy | 4.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 0.4 M AMMONIUM PHOSPHATE |