2UX0
Structure of the oligomerisation domain of calcium-calmodulin dependent protein kinase II gamma
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-12-21 |
| Detector | MARRESEARCH |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 122.954, 158.363, 103.941 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.680 - 2.460 |
| R-factor | 0.186 |
| Rwork | 0.184 |
| R-free | 0.22400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1hkx |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.250 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.700 | 2.560 |
| High resolution limit [Å] | 2.460 | 2.460 |
| Rmerge | 0.060 | 0.460 |
| Number of reflections | 29117 | |
| <I/σ(I)> | 11.4 | 1.45 |
| Completeness [%] | 78.2 | 10.2 |
| Redundancy | 2.63 | 0.13 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 0.1 M SPG PH 7.0, 60% MPD |
