2UVU
Crystal structures of mutant Dpo4 DNA polymerases with 8-oxoG containing DNA template-primer constructs
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 5ID-B |
| Synchrotron site | APS |
| Beamline | 5ID-B |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2006-10-07 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 95.131, 104.124, 52.941 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.260 - 2.700 |
| R-factor | 0.216 |
| Rwork | 0.216 |
| R-free | 0.27000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2uvr |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.300 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.260 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.090 | 0.560 |
| Number of reflections | 14782 | |
| <I/σ(I)> | 14.25 | 2.71 |
| Completeness [%] | 97.9 | 98.9 |
| Redundancy | 5.14 | 5.25 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 8% PEG 3350, 10 MM TRIS-HCL PH 7.5, 50 MM CA(CH3CO2)2, 50MM NACL, 3.5% GLYCEROL |
