2UVQ
Crystal structure of human uridine-cytidine kinase 1 in complex with ADP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-10-19 |
| Detector | MARRESEARCH |
| Spacegroup name | P 62 2 2 |
| Unit cell lengths | 67.919, 67.919, 236.607 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.410 - 3.000 |
| R-factor | 0.197 |
| Rwork | 0.194 |
| R-free | 0.25300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1xrj |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.284 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.3.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.400 | 3.160 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.110 | 0.550 |
| Number of reflections | 12509 | |
| <I/σ(I)> | 19.7 | 4.8 |
| Completeness [%] | 99.5 | 100 |
| Redundancy | 12.1 | 10.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.8-1.0 M SUCCINIC ACID PH 6.7-7.3 |
