2UV0
Structure of the P. aeruginosa LasR ligand-binding domain bound to its autoinducer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7A |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-02-21 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 53.820, 85.330, 75.510 |
| Unit cell angles | 90.00, 95.82, 90.00 |
Refinement procedure
| Resolution | 40.000 - 1.800 |
| R-factor | 0.211 |
| Rwork | 0.209 |
| R-free | 0.25400 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.098 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | CNX |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.090 | 0.580 |
| Number of reflections | 61495 | |
| <I/σ(I)> | 26.4 | 3.1 |
| Completeness [%] | 97.8 | 96.9 |
| Redundancy | 8.1 | 7.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.3 | 20% W/V PEG 4000, 80 MM CALCIUM ACETATE, 40 MM HEPES PH 7.3, 5 MM DTT AND 50 MICROMOLAR N-3-OXO-DODECANOYL-HOMOSERINE LACTONE. |
