2UUH

Crystal structure of Human Leukotriene C4 Synthase in complex with substrate glutathione

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	100
Detector technology	CCD
Detector	MARRESEARCH
Spacegroup name	F 2 3
Unit cell lengths	169.940, 169.940, 169.940
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.030 - 2.150
R-factor	0.185
Rwork	0.183
R-free	0.22000
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.025
RMSD bond angle	2.485
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	51.230
High resolution limit [Å]	1.510	2.150
Rmerge	0.140
Number of reflections	47317
<I/σ(I)>	8.8
Completeness [%]	99.8	100
Redundancy	5	5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1