2USH
5'-NUCLEOTIDASE FROM E. COLI
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | ENRAF-NONIUS FR571 |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-02 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 93.210, 116.290, 132.770 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.220 |
| R-factor | 0.198 |
| Rwork | 0.198 |
| R-free | 0.25400 |
| Structure solution method | MAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 23.600 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.270 |
| High resolution limit [Å] | 2.220 | 2.220 |
| Rmerge | 0.073 * | 0.350 * |
| Total number of observations | 196559 * | |
| Number of reflections | 70373 | |
| <I/σ(I)> | 13 | |
| Completeness [%] | 98.4 | 95.1 |
| Redundancy | 2.8 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.5 * | pH 6.5 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 20 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 5 (mM) | |
| 3 | 1 | drop | 20 (mM) | ||
| 4 | 1 | drop | 0.05 (mM) | ||
| 5 | 1 | reservoir | MES | 100 (mM) | |
| 6 | 1 | reservoir | 15 (mM) | ||
| 7 | 1 | reservoir | 4 (mM) | ||
| 8 | 1 | reservoir | mPEG550 | 20 (%(v/v)) |
