2UAG
UDP-N-ACETYLMURAMOYL-L-ALANINE:D-GLUTAMATE LIGASE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM02 |
| Synchrotron site | ESRF |
| Beamline | BM02 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1997-09 |
| Spacegroup name | P 41 |
| Unit cell lengths | 65.560, 65.560, 136.010 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 1.700 |
| Rwork | 0.180 |
| R-free | 0.20600 |
| Structure solution method | OTHER |
| RMSD bond length | 0.009 |
| RMSD bond angle | 25.400 * |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.100 | 1.800 |
| High resolution limit [Å] | 1.690 | 1.690 |
| Rmerge | 0.081 * | 0.294 * |
| Number of reflections | 61463 | |
| <I/σ(I)> | 36.7 | 9 |
| Completeness [%] | 96.0 | 78.8 |
| Redundancy | 5.5 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 7.5 * | pH 7.0 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | protein | 9.8 (mg/ml) | |
| 10 | 1 | reservoir | 200 (mM) | ||
| 11 | 1 | reservoir | HEPES | 100 (mM) | |
| 2 | 1 | 1 | UMA | 1 (mM) | |
| 3 | 1 | 1 | ADP | 5 (mM) | |
| 4 | 1 | 1 | 1 (mM) | ||
| 5 | 1 | 1 | dithiothreitol | 1 (mM) | |
| 6 | 1 | 1 | HEPES | 20 (mM) | |
| 7 | 1 | reservoir | monodisperse PEG3350 | 14 (%(w/v)) | |
| 8 | 1 | reservoir | 50 (mM) | ||
| 9 | 1 | reservoir | 5 (mM) |
