2TRS
CRYSTAL STRUCTURES OF MUTANT (BETAK87T) TRYPTOPHAN SYNTHASE ALPHA2 BETA2 COMPLEX WITH LIGANDS BOUND TO THE ACTIVE SITES OF THE ALPHA AND BETA SUBUNITS REVEAL LIGAND-INDUCED CONFORMATIONAL CHANGES
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Collection date | 1993-04 |
| Detector | RIGAKU RAXIS IIC |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 184.200, 61.300, 67.900 |
| Unit cell angles | 90.00, 94.50, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.040 |
| R-factor | 0.214 |
| Rwork | 0.214 |
| R-free | 0.29100 * |
| Structure solution method | ISOMORPHOUS WITH PDB ENTRY 1WSY |
| RMSD bond length | 0.014 |
| RMSD bond angle | 23.600 * |
| Data reduction software | RIGAKU |
| Data scaling software | RIGAKU |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 2.130 | |
| High resolution limit [Å] | 2.040 | 2.040 |
| Rmerge | 0.080 | |
| Number of reflections | 37134 | |
| <I/σ(I)> | 8.7 | 2.1 |
| Completeness [%] | 76.9 | 49.4 * |
| Redundancy | 2.54 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 7.8 | pH is adjusted to 7.8 with NaOH * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | Bicine | 50 (mM) | |
| 2 | 1 | 1 | Na-EDTA | 1 (mM) | |
| 3 | 1 | 1 | spermine | 0.8-1.5 (mM) | |
| 4 | 1 | 1 | PEG8000 | 12 (%) | |
| 5 | 1 | 1 | IPP | 0.4 (mM) |
