2TRC
PHOSDUCIN/TRANSDUCIN BETA-GAMMA COMPLEX
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 110 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-06-06 |
| Detector | FUJI |
| Wavelength(s) | 0.9791, 1.7109 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 75.700, 88.510, 98.870 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.400 |
| R-factor | 0.19 |
| Rwork | 0.190 |
| R-free | 0.27700 |
| Structure solution method | MAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 23.500 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELX |
| Refinement software | X-PLOR (3.8) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.440 |
| High resolution limit [Å] | 2.400 | 2.370 |
| Rmerge | 0.060 * | 0.301 * |
| Number of reflections | 24085 | |
| <I/σ(I)> | 16 | 2.9 |
| Completeness [%] | 94.9 | 96.8 * |
| Redundancy | 3 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 4 * | THE PROTEIN COMPLEX (10 MG/ML SOLUTION) WAS CRYSTALLIZED FROM 50 MM SODIUM CITRATE (PH 5.0), 150 MM MAGNESIUM ACETATE, 9.5% PEG 8000, BY HANGING DROP METHOD AT 4 DEGREES C., vapor diffusion - hanging drop, temperature 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 5 (mg/ml) | |
| 2 | 1 | drop | DTT | 0.5 (mM) | |
| 3 | 1 | drop | sodium citrate | 25 (mM) | |
| 4 | 1 | drop | magnesium acetate | 75 (mM) | |
| 5 | 1 | drop | PEG8000 | 4.75 (%) | |
| 6 | 1 | reservoir | sodium citrate | 50 (mM) | |
| 7 | 1 | reservoir | magnesium acetate | 150 (mM) | |
| 8 | 1 | reservoir | PEG8000 | 9.5 (%) |
