2TPL
TYROSINE PHENOL-LYASE FROM CITROBACTER INTERMEDIUS COMPLEX WITH 3-(4'-HYDROXYPHENYL)PROPIONIC ACID, PYRIDOXAL-5'-PHOSPHATE AND CS+ ION
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX9.5 |
| Synchrotron site | SRS |
| Beamline | PX9.5 |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Collection date | 1994-03 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 135.070, 143.910, 59.800 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 - 2.500 |
| Rwork | 0.183 |
| R-free | 0.26300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | TYROSINE PHENOL-LYASE HOLOENZYME |
| RMSD bond length | 0.014 * |
| RMSD bond angle | 0.046 * |
| Data reduction software | DENZO |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | AMoRE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 2.600 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.139 | 0.361 |
| Number of reflections | 33108 | |
| <I/σ(I)> | 4.7 | 1.9 |
| Completeness [%] | 81.0 | 50.3 * |
| Redundancy | 2.7 | 1.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 8 | drop solution is half diluted with a reservoir solution * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 20 (mg/ml) | |
| 2 | 1 | drop | HPPA | 50 (mM) | |
| 3 | 1 | drop | triethanolamine | 50 (mM) | |
| 4 | 1 | drop | dithiothreitol | 1 (mM) | |
| 5 | 1 | drop | PLP | 20000 (M) | |
| 6 | 1 | reservoir | mPEG | 30-45 (%(w/v)) | |
| 7 | 1 | reservoir | 0.15-0.6 (M) | or CsCl |
