2SGD

ASP 18 VARIANT OF TURKEY OVOMUCOID INHIBITOR THIRD DOMAIN COMPLEXED WITH STREPTOMYCES GRISEUS PROTEINASE B AT PH 10.7

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	287
Detector technology	AREA DETECTOR
Detector	SDMS
Spacegroup name	P 1 21 1
Unit cell lengths	45.590, 54.610, 45.620
Unit cell angles	90.00, 119.24, 90.00

Refinement procedure

Resolution	20.000 - 1.800
Rwork	0.196
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3sgb
RMSD bond length	0.025
RMSD bond angle	2.800
Refinement software	TNT

Data quality characteristics

	Overall
Low resolution limit [Å]	20.000
High resolution limit [Å]	1.800
Rmerge	0.105
Number of reflections	17193
Completeness [%]	88.0
Redundancy	1.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		10.7		0.4M SODIUM/POTASSIUM PHOSPHATE BUFFER AT PH 10.7