2SCU

A detailed description of the structure of Succinyl-COA synthetase from Escherichia coli

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-6A
Synchrotron site	Photon Factory
Beamline	BL-6A
Temperature [K]	283
Detector technology	IMAGE PLATE
Collection date	1995-03-01
Spacegroup name	P 43 2 2
Unit cell lengths	98.680, 98.680, 403.760
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 2.300
Rwork	0.195
Structure solution method	MIR
RMSD bond length	0.020
RMSD bond angle	2.042
Data reduction software	WEIS
Data scaling software	BIOMOL ((KBRANI)
Refinement software	TNT (5EB)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.000	2.500
High resolution limit [Å]	2.300	2.300
Rmerge	0.095	0.450
Total number of observations	417950 *
Number of reflections	83411 *
Completeness [%]	92.7
Redundancy	5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Microdialysis *	7.3 *	21 *	Wolodko, W.T., (1984) J. Biol. Chem., 259, 5316. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	enzyme	10 (mg/ml)
2	1	2	ammonium sulfate	1.9 (M)
3	1	2	potassium phosphate	0.1 (M)
4	1	2	dithiothreitol	1 (mM)
5	1	2	CoA	0.1 (mM)
6	1	2	EDTA	0.1 (mM)