2RVE
THE CRYSTAL STRUCTURE OF ECORV ENDONUCLEASE AND OF ITS COMPLEXES WITH COGNATE AND NON-COGNATE DNA SEGMENTS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | ELLIOTT GX-21 |
| Detector technology | AREA DETECTOR |
| Detector | SIEMENS-NICOLET X100 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 68.500, 79.600, 66.400 |
| Unit cell angles | 90.00, 104.60, 90.00 |
Refinement procedure
| Resolution | 6.000 - 3.000 |
| R-factor | 0.178 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.750 * |
| Refinement software | TNT |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 2.800 * |
| Rmerge | 0.047 * |
| Total number of observations | 32155 * |
| Number of reflections | 17027 * |
| Completeness [%] | 99.0 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6 | Winkler, F.K., (1991) J.Mol.Biol., 217, 235. * |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | WATER | ||
| 2 | 1 | 1 | PEG 4000 | ||
| 3 | 1 | 1 | NACL | ||
| 4 | 1 | 2 | WATER | ||
| 5 | 1 | 2 | MES | ||
| 6 | 1 | 2 | NACL |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | enzyme | 7-10 (mg/ml) | |
| 2 | 1 | drop | ammonium sulfate | ||
| 3 | 1 | reservoir | sodium potassium phosphate | 10-20 (mM) | |
| 4 | 1 | reservoir | 250 (mM) | ||
| 5 | 1 | reservoir | oligonucleotide | 2-6 (mg/ml) |
