2RTM

STREPTAVIDIN-2-IMINOBIOTIN-SULFATE COMPLEX, PH 3.50, SPACE GROUP I4122

Experimental procedure

Detector technology	IMAGE PLATE
Detector	RIGAKU
Spacegroup name	I 41 2 2
Unit cell lengths	58.170, 58.170, 174.460
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	? - 1.330 *
R-factor	0.199
Rwork	0.199
R-free	0.23800
RMSD bond length	0.017
RMSD bond angle	24.700 *
Data reduction software	bioteX ((MSC))
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]		1.650
High resolution limit [Å]	1.330 *	1.580
Rmerge	0.045
Total number of observations	63959 *
Number of reflections	28990
Completeness [%]	65.0	31.7
Redundancy	2.2	2.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	4.5 *	20 *	Pahler, A., (1987) J. Biol. Chem., 262, 13933. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10-20 (mg/ml)
2	1	drop	potassium acetate	50 (mM)
3	1	drop		200 (mM)
4	1	reservoir	ammonium sulfate	30 (%sat)